【研究亮點】利用小鼠頷面疼痛行為模型研究低能量雷射止痛效應和大麻素與組織胺系統 於低能量雷射止痛之角色_許昇峰副教授

【利用小鼠頷面疼痛行為模型研究低能量雷射止痛效應和大麻素與組織胺系統

於低能量雷射止痛之角色】

The interactions between analgesic effects of low-level laser, Cannabinoid-dependent analgesia and Histamine system in a mouse model of orofacial pain

中文摘要

        疼痛是大腦皮質針對感覺訊號的解讀，提醒身體注意組織或器官損傷。當疼痛發生於口面部，稱之為頷面疼痛，具有多樣且複雜的原因。其中由於咀嚼肌和顳下頷關節異常造成的顳下頷關節障礙，是最常見的非牙源性慢性口面部疼痛原因。顳下頷關節障礙可分為咀嚼肌群障礙以及關節相關障礙，治療上通常以處理關節脫位及肌肉關節炎症為主。然而，顳下頷關節障礙帶來的疼痛、咬合、語言、社會心理方面的負擔，以及病因的診斷困難，造成其治療方式的多樣化。

        電針由於其有效的止痛效應，被應用於頷面疼痛的治療中。且透過小鼠頷面疼痛模型實驗中發現，療效在大麻素系統較為優勢，並且和第一型大麻受體拮抗劑以及組織胺途徑有關。而低能量雷射作為頷面疼痛的治療方式之一，其機轉仍不明朗，將探討是否與電針具有類似止痛機轉。

        預試驗中，在小鼠頷面疼痛模型，以低能量雷射介入，使用地倉穴(ST4)和頰車穴(ST6)作為穴位點，對於由弗氏佐劑(CFA，1µg/10µl熱滅活的分枝桿菌或食鹽水)注射左邊咀嚼肌引發之疼痛進行治療。實驗測定痛覺的方式採用馮佛雷纖維細絲痛覺測試(von-Frey test)，利用von-Frey test接觸注射試劑處，測定小鼠頷面部疼痛閾值。發現小鼠頷面疼痛模型中低能量雷射可以緩解疼痛，將以藥物及組織分析做進一步探討。

關鍵詞：頷面疼痛、雷射針灸、大麻素系統、組織胺系統

ABSTRACT

Pain is the interpretation of sensory signals by the cerebral cortex, reminding the body of tissue or organ damage. When the pain occurs in the orofacial region, superior from the neck, anterior from the auricular foramen, inferior from the orbitomeatal line and in the oral cavity, it is called orofacial pain, which has multiple and complex causes. Among them, the temporomandibular joint disorder caused by the disorders of the masticatory muscles and the temporomandibular joint is the most common cause of non-odontogenic chronic orofacial pain. Temporomandibular joint disorders can be divided into masticatory muscle disorders and joint-related disorders. The treatment usually focuses on joint dislocation and inflammation of muscles or joint. However, the pain, occlusion disorders, language impairment, and socio-psychological disability caused by temporomandibular joint disorder, as well as the difficulty in diagnosis of the cause, have caused the diversification of its treatment methods.

    Electroacupuncture is used in the treatment of orofacial pain due to its effective analgesic effect. Through the mouse orofacial pain model experiment, it is found that the analgesic effect is more dominant in the cannabinoid pathways, and it is also correlated by the first type cannabinoid receptor antagonist and histamine pathway. However, the mechanism of low-level laser, as one of the treatments for orofacial pain is still unclear. We will study whether it has a similar mechanism of pain relief with electroacupuncture.

    In our pre-test, in the mouse orofacial pain model, low-level laser treatment is used to use Dicang (ST4) and Jiache (ST6) as acupoints to treat the myofascial pain caused by the complete Freund's adjuvant (CFA, 1μg/10μl) Heat-inactivated mycobacterium or saline) injected into the left masticatory muscles. Our method of measuring pain is the von-Frey test, and it is used to contact the injection region to measure the pain threshold of the mouse's orofacial area. It is found that low-level laser treatment in the mouse orofacial pain model can alleviate the pain. We will further study the mechanism of low-level laser treatment in the mouse orofacial pain model by pharmacological and molecular biological analysis.

Keywords：orofacial pain, low-level laser, cannabinoid pathway, histamine pathway